Smekens L, Golstein J, Vanhaelst L
J Endocrinol Invest. 1983 Apr;6(2):113-7. doi: 10.1007/BF03350582.
The deiodination of T4 to T3 has been studied after incubation of whole lymphocytes with different T4 concentrations, followed by a T3 radioimmunological determination performed on unextracted samples. The lymphocytes were isolated from blood from normal volunteers of both sexes and different age classes as well as from myxedematous subjects and patients with low T3 syndrome. The T3 production, in normal subjects, expressed as pmol/10.10(6) cells was 0.41 +/- 0.75 (T4 = 4.10(-7) M, n = 12), 1.43 +/- 0.14 (T4 = 4.10(-6) M, n = 26) and 1.65 +/- 0.29 (T4 = 8.10(-6) M, n = 11). There was no sex or age-related difference. In 3 myxedematous patients the T3 production was lower than the corresponding value obtained in normal subjects. The T3 production in patients with low T3 syndrome was lower than in controls (p less than 0.001); an inverse correlation was found between the T3 production and the serum rT3 levels, whereas no correlation could be found with serum T3 levels.
在用不同浓度的T4孵育全淋巴细胞后，接着对未提取样本进行T3放射免疫测定，以此研究T4向T3的脱碘作用。淋巴细胞取自不同年龄组的正常男女志愿者的血液，以及黏液水肿患者和低T3综合征患者的血液。在正常受试者中，以pmol/10.10(6)细胞表示的T3生成量为0.41±0.75（T4 = 4.10(-7) M，n = 12）、1.43±0.14（T4 = 4.10(-6) M，n = 26）和1.65±0.29（T4 = 8.10(-6) M，n = 11）。不存在与性别或年龄相关的差异。在3名黏液水肿患者中，T3生成量低于正常受试者的相应值。低T3综合征患者的T3生成量低于对照组（p<0.001）；发现T3生成量与血清反T3水平呈负相关，而与血清T3水平未发现相关性。
