Hasegawa H, Ushio Y, Hayakawa T, Yamada K, Mogami H
J Neurosurg. 1983 Aug;59(2):304-10. doi: 10.3171/jns.1983.59.2.0304.
An experimental model for blood-borne cerebral metastases was developed by introducing Walker 256 carcinoma cells selectively into the intracranial internal carotid artery of rats. This model was used to study the regional capillary permeability of rat brain and metastatic brain tumors of various sizes with the aid of 14C alpha-aminoisobutyric acid (AIB) quantitative autoradiography. The regional capillary permeability varied with the anatomical location and size of the tumor. Intraparenchymal tumors less than 1 mm in diameter showed no increased permeability to AIB. As the tumors enlarged over 1 mm in diameter, the permeability in the intraparenchymal tumors increased proportionally, but remained less than one-third of capillary permeability of subcutaneously transplanted tumors. Capillary permeability in the peripheral invasive part and necrotic center was less than in the viable part of large tumors. Capillary permeability in metastatic tumors of the choroid plexus and meninges was significantly higher than in tumors of the brain parenchyma. The results suggest that the uptake of chemotherapeutic agents that do not cross the blood-brain barrier easily varies with the anatomical location and size of the metastatic tumors.
通过将Walker 256癌细胞选择性地注入大鼠颅内颈内动脉，建立了血行性脑转移的实验模型。利用14Cα-氨基异丁酸（AIB）定量放射自显影技术，该模型被用于研究大鼠脑的区域毛细血管通透性以及不同大小的转移性脑肿瘤。区域毛细血管通透性随肿瘤的解剖位置和大小而变化。直径小于1mm的脑实质内肿瘤对AIB的通透性未增加。随着肿瘤直径增大超过1mm，脑实质内肿瘤的通透性成比例增加，但仍低于皮下移植肿瘤毛细血管通透性的三分之一。大肿瘤外周浸润部分和坏死中心的毛细血管通透性低于存活部分。脉络丛和脑膜转移性肿瘤的毛细血管通透性明显高于脑实质肿瘤。结果表明，不易穿过血脑屏障的化疗药物的摄取随转移性肿瘤的解剖位置和大小而变化。
