Brown D M, Steffes M W, Thibert P, Azar S, Mauer S M
Metabolism. 1983 Jul;32(7 Suppl 1):131-5. doi: 10.1016/s0026-0495(83)80026-2.
Morphologic characteristics of the renal glomeruli and tubules of BB rats with spontaneous diabetes mellitus were studied at 30 weeks' duration of diabetes. Whereas the glomerular basement membrane (GBM) was significantly thickened, no changes in the diabetic glomeruli were seen in the peripheral capillary wall area and in the fractional volumes of the mesangial cells or of the mesangial matrix. Light microscopy of the diabetic kidneys were normal, and immunofluorescent examination of diabetic glomeruli showed no increased accumulation of albumin, C3, or IgG. Diabetic rats had increased renal blood flow and glomerular filtration rates. Diabetic rats at 7, 17, and 30 weeks excreted normal amounts of urinary albumin. Thus kidneys of the BB diabetic rat differ from other experimental models of diabetes in that GBM thickening occurs in the absence of mesangial changes and of increased albuminuria. These studies suggest that the mesangium may influence glomerular permeability in diabetes, while thickening of the GBM in diabetes does not necessarily coincide with increased urinary albumin excretion. Furthermore, these results are consonant with the hypothesis that genetic factors may influence the pathological expression of diabetic nephropathy in rats.
对病程达30周的自发性糖尿病BB大鼠的肾小球和肾小管的形态学特征进行了研究。虽然肾小球基底膜（GBM）显著增厚，但在糖尿病肾小球的外周毛细血管壁区域以及系膜细胞或系膜基质的分数体积方面未见变化。糖尿病肾脏的光学显微镜检查结果正常，糖尿病肾小球的免疫荧光检查显示白蛋白、C3或IgG没有增加的积聚。糖尿病大鼠的肾血流量和肾小球滤过率增加。7周、17周和30周的糖尿病大鼠尿白蛋白排泄量正常。因此，BB糖尿病大鼠的肾脏与其他糖尿病实验模型不同，在于GBM增厚发生时系膜无变化且蛋白尿未增加。这些研究表明，系膜可能影响糖尿病时的肾小球通透性，而糖尿病时GBM增厚不一定与尿白蛋白排泄增加同时出现。此外，这些结果与遗传因素可能影响大鼠糖尿病肾病病理表现的假说一致。
