Salih M M, Ernø H, Simonsen V
Acta Vet Scand. 1983;24(1):14-33. doi: 10.1186/BF03546754.
The purpose of this paper is to further characterize and determine relatedness among some Mycoplasma species or serogroups of significant practical veterinary interest. Twenty-four strains were examined for the presence of 35 enzymes by horizontal starch gel electrophoresis, revealing a total of 127 different electromorphs of 30 enzymes. Inter- as well as intraspecific differences were found demonstrating the application of isoenzyme studies in classification as well as epidemiology. It is concluded that the F38 group of and group 7 of constitute 2 new species, The elevation of the 2 subspecies of M. mycoides (mycoides and capri) to species level is favoured, but it is suggested that a decision be taken internationally, considering the practical consequences of a given nomenclature. Three alternative possibilities for classification are presented. Regarding identification the results suggest that the presence or absence of maltase and ornithine transcarbamylase indicate whether an isolate is related to the agent of contagious bovine pleuropneumonia or merely a representative of either the caprine LG type of M. mycoides subsp. mycoides or the classical caprine subspecies, M. mycoides subsp. capri.
本文的目的是进一步描述和确定一些具有重要实际兽医意义的支原体物种或血清群之间的相关性。通过水平淀粉凝胶电泳对24株菌株进行了35种酶的检测，共发现30种酶的127种不同电泳形态。种间和种内差异均有发现，证明了同工酶研究在分类和流行病学中的应用。结论是，F38群和群7构成2个新物种，支持将丝状支原体（丝状亚种和山羊亚种）的2个亚种提升到物种水平，但建议在国际上做出决定时，考虑特定命名法的实际后果。提出了三种分类的替代可能性。关于鉴定，结果表明麦芽糖酶和鸟氨酸转氨甲酰酶的有无表明分离株是与传染性牛胸膜肺炎病原体相关，还是仅仅是丝状支原体丝状亚种山羊LG型或经典山羊亚种丝状支原体山羊亚种的代表。
