Lanham S M, Lemcke R M, Scott C M, Grendon J M
J Gen Microbiol. 1980 Mar;117(1):19-31. doi: 10.1099/00221287-117-1-19.
The reference strains Type A and Type B and two equine strains of Acholeplasma laidlawii were examined for a wide range of isoenzymes using thin-layer starch-gel electrophoresis; in addition two isoenzymes were examined in two strains of A. equifetale. The type strains A and B of A. laidlawii were differentiated by their lactate dehydrogenase, phosphoglucomutase and aspartate aminotransferase patterns and the two equine strains by their hexokinase, lactate dehydrogenase and phosphoglycerate kinase patterns. The two pairs of strains differed from one another with respect to hexokinase, phosphoglucomutase, adenylate kinase and glucose-6-phosphate dehydrogenase. The two strains of A. equifetale could be distinguished by their isoenzymes of hexokinase. The two species were differentiated by their hexokinase and phosphoglucomutase patterns.
使用薄层淀粉凝胶电泳对参考菌株A型和B型以及两株莱氏无胆甾原体马菌株进行了多种同工酶检测；此外，还对两株马胎儿无胆甾原体菌株中的两种同工酶进行了检测。莱氏无胆甾原体的A型和B型菌株可通过其乳酸脱氢酶、磷酸葡萄糖变位酶和天冬氨酸氨基转移酶图谱进行区分，两株马菌株可通过其己糖激酶、乳酸脱氢酶和磷酸甘油酸激酶图谱进行区分。这两对菌株在己糖激酶、磷酸葡萄糖变位酶、腺苷酸激酶和葡萄糖-6-磷酸脱氢酶方面彼此不同。两株马胎儿无胆甾原体菌株可通过其己糖激酶同工酶进行区分。这两个物种可通过其己糖激酶和磷酸葡萄糖变位酶图谱进行区分。
