Thiermann A B, Garrett L A
Am J Vet Res. 1983 May;44(5):884-7.
An enzyme-linked immunosorbent assay (ELISA), which used the phenol phase of a hot phenol-water extraction as antigen, and the microscopic agglutination test were compared for the detection of antibodies to Leptospira interrogans serovars hardjo and pomona in cattle. Among serum samples from vaccinated cattle, the ELISA (using antiglobulin) was positive to 64% with hardjo antigen and to 78% with pomona antigen, whereas the microscopic agglutination test was positive to only 18% with hardjo and 23% with pomona. There were no ELISA reactions observed among known negative-control samples. The ELISA, using antiglobulin conjugate, was overall a more sensitive technique for the detection of leptospiral antibodies in cattle.
将热酚-水提取法的酚相作为抗原的酶联免疫吸附测定（ELISA）和显微镜凝集试验，被用于比较检测牛体内问号钩端螺旋体哈焦血清型和波摩那血清型的抗体。在接种疫苗的牛的血清样本中，ELISA（使用抗球蛋白）检测哈焦抗原时阳性率为64%，检测波摩那抗原时阳性率为78%，而显微镜凝集试验检测哈焦抗原时阳性率仅为18%，检测波摩那抗原时阳性率为23%。在已知的阴性对照样本中未观察到ELISA反应。使用抗球蛋白缀合物的ELISA总体上是检测牛体内钩端螺旋体抗体更敏感的技术。
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