Fairbrother J M
J Clin Microbiol. 1984 Dec;20(6):1089-93. doi: 10.1128/jcm.20.6.1089-1093.1984.
The serological interrelationship of a sonicated antigen (POM-S) and an alkali-extracted "fraction 4" antigen (POM-F4) from Leptospira interrogans serovar pomona, and an ethanol-precipitated (PAT-E) and a formolized, sonicated antigen (PAT-F) from Leptospira biflexa serovar patoc were investigated. The serological responses of rabbits immunized with these antigens were examined by the enzyme-linked immunosorbent assay (ELISA), the microscopic agglutination test, and the 2-mercaptoethanol microscopic agglutination test. Antisera from these rabbits absorbed by the homologous or heterologous antigens were examined by the ELISA. The PAT-E and PAT-F antigens were genus specific and were serologically closely related but not identical. Similarly, the POM-F4 and POM-S antigens showed some serological relatedness. By the use of absorbed antisera, the serovar pomona-derived antigens were shown to be serologically related to the PAT-F but not to the PAT-E antigen in the ELISA. It is suggested that the use of several such antigens in the ELISA may reveal differences in the kinetics of the antibody response in animals infected by different leptospiral serovars.
研究了问号钩端螺旋体波摩那群的超声处理抗原（POM - S）和碱提取的“4组分”抗原（POM - F4），以及双曲钩端螺旋体帕托群的乙醇沉淀抗原（PAT - E）和甲醛处理的超声抗原（PAT - F）之间的血清学相互关系。用酶联免疫吸附测定（ELISA）、显微镜凝集试验和2 - 巯基乙醇显微镜凝集试验检测用这些抗原免疫的家兔的血清学反应。用ELISA检测这些家兔被同源或异源抗原吸收后的抗血清。PAT - E和PAT - F抗原具有属特异性，在血清学上密切相关但不相同。同样，POM - F4和POM - S抗原也显示出一定的血清学相关性。在ELISA中，通过使用吸收后的抗血清表明，波摩那群衍生的抗原与PAT - F血清学相关，但与PAT - E抗原无关。建议在ELISA中使用几种这样的抗原可能会揭示感染不同钩端螺旋体血清型的动物抗体反应动力学的差异。
