Mantle P G, Perera K P, Maishman N J, Mundy G R
Appl Environ Microbiol. 1983 May;45(5):1486-90. doi: 10.1128/aem.45.5.1486-1490.1983.
Roquefortine and the penitrems were biosynthesised concurrently at an approximately equimolar rate by Penicillium crustosum after growth and sporulation. [14C]mevalonic acid was incorporated (15% efficiency) into the isoprenoid regions of the penitrem and roquefortine molecules to an extent consistent with their 6:1 molar ratio of isoprenoid components. [14C]penitrem A (specific activity, 3.4 X 10(2) mu Ci mmol-1) and 14C-penitrems B, C, and E readministered to young cultures were metabolically interconverted, indicating considerable metabolic flux, though generally directed towards penitrem A as the end product and suggesting a metabolic grid for the penitrem metabolites. Addition of bromide to the medium preferentially favored the production of bromo-analogs rather than the usual chloropenitrems.
在生长和产孢后，皮壳青霉以近似等摩尔的速率同时生物合成了罗克福汀和青霉震颤素。[14C]甲羟戊酸以15%的效率掺入到青霉震颤素和罗克福汀分子的类异戊二烯区域，掺入程度与其类异戊二烯成分6:1的摩尔比一致。重新施用于年轻培养物的[14C]青霉震颤素A（比活度为3.4×10(2) μCi mmol-1）以及14C-青霉震颤素B、C和E发生了代谢互变，这表明存在相当大的代谢通量，尽管通常以青霉震颤素A作为终产物，这暗示了青霉震颤素代谢物的代谢网格。向培养基中添加溴化物优先促进了溴代类似物的产生，而不是通常的氯代青霉震颤素。
