Nishida T, Ohashi Y, Awata T, Manabe R
Arch Ophthalmol. 1983 Jul;101(7):1046-8. doi: 10.1001/archopht.1983.01040020048007.
Fibronectin, a glycoprotein, is present in plasma and extracellular matrix and is responsible for cellular adhesion. With the use of affinity chromatography, we purified plasma fibronectin from two patients with trophic corneal ulcer and persistent epithelial defect after conventional therapy was ineffective. Reepithelialization began three days after initiation of treatment with autologous purified fibronectin eyedrops, and the epithelial defects disappeared completely within three weeks. No recurrence was observed after treatment ended. By modifying the original chromatographic procedure, we were able to obtain purified fibronectin in two hours, which allowed us to treat outpatients as well as inpatients.
纤连蛋白是一种糖蛋白，存在于血浆和细胞外基质中，负责细胞黏附。通过亲和层析法，我们从两名常规治疗无效的营养性角膜溃疡和持续性上皮缺损患者的血浆中纯化出了纤连蛋白。在用自体纯化的纤连蛋白滴眼液治疗开始三天后，角膜上皮开始再生，上皮缺损在三周内完全消失。治疗结束后未观察到复发情况。通过改进原始的层析方法，我们能够在两小时内获得纯化的纤连蛋白，这使得我们既可以治疗门诊患者，也可以治疗住院患者。
