通过高效液相色谱法分离卟啉异构体。
Separation of porphyrin isomers by high-performance liquid chromatography.
作者信息
Lim C K, Rideout J M, Wright D J
出版信息
Biochem J. 1983 May 1;211(2):435-8. doi: 10.1042/bj2110435.
Abstract
A reversed-phase gradient elution system is described for the simultaneous separation of the type I and type III isomers of 8-, 7-, 6-, 5- and 4-carboxylated porphyrins and isocoproporphyrins. The method, adaptable for isocratic and stepwise separation of individual groups of isomers, is also suitable for preparative isolation of pure porphyrins. The analyses of porphyrin isomers in the urine and faeces of porphyric patients are examples of applications.
摘要
描述了一种反相梯度洗脱系统，用于同时分离8-、7-、6-、5-和4-羧基化卟啉及异科普卟啉的I型和III型异构体。该方法适用于异构体各单独组的等度和逐步分离，也适用于纯卟啉的制备分离。卟啉病患者尿液和粪便中卟啉异构体的分析就是应用实例。
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