通过二维凝胶电泳揭示的多头绒泡菌的一种细胞周期依赖性蛋白。
A cell-cycle-dependent protein of Physarum polycephalum revealed by two-dimensional gel electrophoresis.
作者信息
Pahlic M, Tyson J J
出版信息
Biochem J. 1983 Apr 15;212(1):245-7. doi: 10.1042/bj2120245.
Abstract
Silver-stained two-dimensional polyacrylamide gels of Physarum polycephalum extracts have revealed no major changes in protein accumulation during the synchronous nuclear division cycle. However, one protein, or apparent Mr 32 000 and isoelectric point 4.9, shows a reproducible ten-fold increase in total amount between early G2 phase and metaphase. This protein represents about 0.005% of total plasmodial protein.
摘要
多头绒泡菌提取物的银染二维聚丙烯酰胺凝胶显示，在同步核分裂周期中蛋白质积累没有重大变化。然而，一种表观分子量为32000且等电点为4.9的蛋白质，在G2期早期到中期之间总量呈现出可重复的10倍增加。这种蛋白质约占原生质体总蛋白的0.005%。
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