Elliott S G, McLaughlin C S
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4384-8. doi: 10.1073/pnas.75.9.4384.
Centrifugal elutriation was used to separate cells of Saccharomyces cerevisiae in balanced exponential growth according to position in the cell cycle. Macromolecular synthesis was examined. DNA synthesis was found to be periodic, but RNA and protein synthesis showed an exponential increase in rate. Two-dimensional electrophoresis was used to determine the rate of synthesis of individual proteins, with 111 of the more abundant cellular proteins selected for analysis from among the more than 1000 proteins that migrate in the system. All the examined proteins showed an exponentially increasing rate of synthesis.
采用离心淘析法，根据细胞周期所处位置，分离处于平衡指数生长期的酿酒酵母细胞。检测了大分子合成情况。发现DNA合成具有周期性，但RNA和蛋白质合成速率呈指数增加。利用二维电泳确定单个蛋白质的合成速率，从该系统中迁移的1000多种蛋白质中挑选出111种含量较高的细胞蛋白质进行分析。所有检测的蛋白质合成速率均呈指数增加。
