通过一种新的检测系统对平板凝胶中3H标记的蛋白质和RNA进行定位与直接定量分析。
Localization and direct quantitation of 3H-labeled proteins and RNAs in slab gels by a new detection system.
作者信息
Kruppa J
出版信息
Biochem Biophys Res Commun. 1983 Jun 15;113(2):703-9. doi: 10.1016/0006-291x(83)91783-7.
The distribution patterns of 3H-labeled proteins and RNAs in dehydrated polyacrylamide and agarose gels have been recorded with a newly developed instrument: the Linear Analyzer which has a position-sensitive detector that counts a whole gel lane simultaneously. The high sensitivity makes the Linear Analyzer a suitable instrument for direct quantitation of tritium labeled macromolecules in gels without pretreatment by impregnating reagents. The very low detection limit of the Linear Analyzer reduces substantially the time of evaluation in comparison to the relatively long exposure times needed in fluorography. The resolution of radioactivity profiles monitored by the Linear Analyzer reaches the quality attained by fluorography.
利用一种新开发的仪器——线性分析仪，记录了脱水聚丙烯酰胺凝胶和琼脂糖凝胶中3H标记蛋白质和RNA的分布模式。该线性分析仪配有一个位置敏感探测器，可同时对整个凝胶泳道进行计数。高灵敏度使线性分析仪成为一种无需用浸渍试剂预处理就能直接定量凝胶中氚标记大分子的合适仪器。与荧光自显影所需的相对较长曝光时间相比，线性分析仪极低的检测限大大缩短了评估时间。由线性分析仪监测的放射性图谱分辨率达到了荧光自显影的质量水平。
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