人生长激素对淋巴细胞增殖的调节作用。
Modulation of lymphoproliferation by human growth hormone.
作者信息
Kiess W, Holtmann H, Butenandt O, Eife R
出版信息
Eur J Pediatr. 1983 Mar;140(1):47-50. doi: 10.1007/BF00661904.
This study was designed to investigate the capacity of human growth hormone (hGH) to induce proliferation of human peripheral blood lymphocytes (PBL) and its effect on lectin-induced lymphoproliferation, as measured by [3H]-thymidine (3H-TdR) incorporation into lymphocyte DNA. We observed that hGH itself induced lymphoproliferation. Maximal responses were reached with 50 ng/ml after 4 days and 25 ng/ml after 7 days. Anti-hGH-antiserum completely blocked the stimulatory capacity of hGH. Further evidence for the specificity of the effect was obtained by the finding that no other hormone present in the hGH preparation produced any effect on lymphocyte cultures. In contrast to its stimulatory capacity, hGH reduced the proliferative response of PBL to phytohaemagglutinin (PHA). This effect was also neutralized by anti-hgH-antiserum. These results show a direct stimulatory effect of hGH on PBL and a regulatory effect of the hormone on lectin-induced lymphoproliferation.
本研究旨在探讨人生长激素（hGH）诱导人外周血淋巴细胞（PBL）增殖的能力及其对凝集素诱导的淋巴细胞增殖的影响，通过[3H] - 胸腺嘧啶核苷（3H - TdR）掺入淋巴细胞DNA来测定。我们观察到hGH本身可诱导淋巴细胞增殖。4天后50 ng/ml达到最大反应，7天后25 ng/ml达到最大反应。抗hGH抗血清完全阻断了hGH的刺激能力。通过发现hGH制剂中存在的其他激素对淋巴细胞培养物无任何影响，进一步获得了该效应特异性的证据。与其刺激能力相反，hGH降低了PBL对植物血凝素（PHA）的增殖反应。这种效应也被抗hGH抗血清中和。这些结果表明hGH对PBL有直接刺激作用，且该激素对凝集素诱导的淋巴细胞增殖有调节作用。
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