Mercola K E, Cline M J, Golde D W
Blood. 1981 Aug;58(2):337-40.
In order to investigate the effect of human growth hormone on T lymphocytes, we utilized a clonogenic assay for mitogen-responsive human peripheral blood T lymphocytes. Lymphocytes were purified by density gradient centrifugation and incubated in the presence of phytohemagglutinin using a two-layer agar technique for CFU- T-lymphocyte culture. Nanogram concentrations of human growth hormone, ovine prolactin, human chorionic somatomammotropin, or growth hormone fragment were added to cell cultures. Growth hormone potentiated normal T-cell colony formation in a species-specific manner. Cells from a homogeneous T-lymphoblast line derived from a patient with a T-cell variant of hairy cell leukemia also showed augmentation of colony growth in the presence of human growth hormone. These studies provide evidence for a direct effect of growth hormone on normal and some neoplastic human T cells.
为了研究人生长激素对T淋巴细胞的作用，我们采用了一种针对有丝分裂原反应性人外周血T淋巴细胞的克隆形成试验。通过密度梯度离心法纯化淋巴细胞，并使用两层琼脂技术在植物血凝素存在的情况下进行培养，用于CFU - T淋巴细胞培养。将纳克浓度的人生长激素、羊催乳素、人绒毛膜生长催乳素或生长激素片段添加到细胞培养物中。生长激素以种属特异性方式增强正常T细胞集落形成。来自一名毛细胞白血病T细胞变异型患者的同质T淋巴母细胞系的细胞，在人生长激素存在的情况下也显示出集落生长增加。这些研究为生长激素对正常和一些肿瘤性人T细胞的直接作用提供了证据。
