Neurohr K J, Gollin G, Barrett E J, Shulman R G
FEBS Lett. 1983 Aug 8;159(1-2):207-10. doi: 10.1016/0014-5793(83)80447-5.
31P NMR spectra of heart in-situ in live guinea pigs were obtained continuously in 20.5 s time blocks during 3 min of anoxia, during subsequent reoxygenation and, in separate animals, during terminal anoxia. Reversible anoxia resulted in rapid degradation of phosphocreatine (t 1/2 = 54.5 +/- 2.5 s) which recovered fully during reoxygenation. Heart Pi increased during anoxia and returned to basal levels after oxygen was restored. During 3 min of anoxia, no significant changes in ATP levels or pH were detected. The results demonstrate that it is feasible to measure rapid fluxes of high energy phosphates by 31P NMR in intact animals during and after anoxic stress to the myocardium.
在活的豚鼠心脏原位情况下，在缺氧3分钟期间、随后的复氧过程中以及在单独的动物中进行终末期缺氧时，以20.5秒的时间间隔连续获取31P NMR光谱。可逆性缺氧导致磷酸肌酸迅速降解（t1/2 = 54.5 +/- 2.5秒），在复氧过程中完全恢复。心脏无机磷酸盐在缺氧期间增加，在恢复氧气后恢复到基础水平。在缺氧3分钟期间，未检测到ATP水平或pH值有显著变化。结果表明，在心肌缺氧应激期间和之后，通过31P NMR测量完整动物体内高能磷酸盐的快速通量是可行的。
