Clifford P S, Coon R L, von Colditz J H, Zuperku E J, Kampine J P
J Appl Physiol Respir Environ Exerc Physiol. 1983 Jun;54(6):1451-6. doi: 10.1152/jappl.1983.54.6.1451.
To produce a chronically lung-denervated animal model, a single-stage surgical procedure was performed on five beagle dogs. A left thoracotomy allowed hilar stripping of the pulmonary contributions to the left vagus nerve and transection of the right vagal trunk. The criterion for denervation was defined as the absence of the Hering-Breuer reflex (HBR). The five denervated dogs (DD) as well as five control dogs (CD) were evaluated for presence of the HBR under pentobarbital sodium (Nembutal, 30 mg/kg) anesthesia. Between the 3rd and 8th wk postsurgery, the HBR was clearly abolished in the DD but present in the CD. By the 12th-14th wk postsurgery, the HBR was again present in the DD. This relatively uncomplicated surgical procedure effectively produced a lung-denervated animal model. However, reinnervation occurred relatively soon, thus demonstrating the importance of regular frequent evaluation of the HBR when using this and any other surgically produced pulmonary denervation model.
为建立慢性肺去神经支配动物模型，对5只比格犬实施了单阶段手术操作。通过左胸切开术，在肺门处剥离左迷走神经的肺分支，并切断右迷走神经干。去神经支配的标准定义为无赫林-布雷尔反射（HBR）。在戊巴比妥钠（戊巴比妥，30mg/kg）麻醉下，评估5只去神经支配犬（DD）和5只对照犬（CD）是否存在HBR。术后第3至8周，DD组的HBR明显消失，而CD组存在该反射。术后第12至14周，DD组再次出现HBR。这种相对简单的手术操作有效地建立了肺去神经支配动物模型。然而，再支配出现得相对较快，因此证明了在使用该模型及任何其他手术制作的肺去神经支配模型时，定期频繁评估HBR的重要性。
