Leprán I, Koltai M, Siegmund W, Szekeres L
J Pharmacol Methods. 1983 May;9(3):219-30. doi: 10.1016/0160-5402(83)90041-4.
A method for studying the acute phase of myocardial infarction in conscious rats has been developed. In preliminary surgery, a loose ligature of atraumatic silk was understitched around the left coronary artery. Its ends were pulled through a polyethylene tube placed within the thorax and fixed under the skin. Seven days later, coronary occlusion was performed by tightening the ligature in conscious animal. Lidocaine and pindolol pretreatment increased the survival rate and attenuated the life-threatening arrhythmias during the first 20 min, but did not influence the infarct size 16 hrs later. An ex vivo perfusion technique for determining the ischemic area has also been developed. 3, 6, and 20 min after coronary ligation, the hearts were excised and perfused with 4% formaldehyde. The ischemic area could not be perfused and remained dark red with a sharp border-line. At the 3rd and 20th min its size was as same as that of the 16-hr infarcted area; however at the 6th min it increased by 50%. Lidocaine and pindolol eliminated this transitory increase. These methods appear to be valuable for large-scale determination of drug effects on the acute phase of experimental myocardial infarction in conscious rats, and for estimating their action on the size of ischemic area very early after coronary occlusion.
已开发出一种在清醒大鼠中研究心肌梗死急性期的方法。在初步手术中，用无创伤丝线在左冠状动脉下方进行宽松结扎。其两端穿过置于胸腔内的聚乙烯管并固定于皮下。7天后，通过在清醒动物中收紧结扎线来进行冠状动脉闭塞。利多卡因和吲哚洛尔预处理提高了存活率，并减轻了最初20分钟内危及生命的心律失常，但16小时后对梗死面积无影响。还开发了一种用于确定缺血区域的离体灌注技术。冠状动脉结扎后3、6和20分钟，取出心脏并用4%甲醛灌注。缺血区域无法被灌注，仍呈深红色，边界清晰。在第3分钟和第20分钟时，其大小与16小时梗死区域相同；然而在第6分钟时，其面积增加了50%。利多卡因和吲哚洛尔消除了这种短暂增加。这些方法对于大规模测定药物对清醒大鼠实验性心肌梗死急性期的影响，以及估计冠状动脉闭塞后早期药物对缺血区域大小的作用似乎很有价值。
