Hädge D, Ambrosius H
Mol Immunol. 1983 Jun;20(6):597-606. doi: 10.1016/0161-5890(83)90004-4.
Studies on comparison of antigenic relationships between the immunoglobulin of chicken bile--the so-called chicken IgA--and human, porcine, as well as murine IgA were performed by a series of double-antibody and solid-phase RIA systems using H chain-specific anti-chicken biliary Ig sera and alpha chain-specific anti-human IgA sera produced in rabbits and in carp. It was not possible to demonstrate a significant immunological cross-reactivity of the Ig of chicken bile to the Ig's of IgA class. Besides, we could not find an Ig type similar to this chicken biliary Ig in pooled normal sera or in the crude Ig fractions precipitated by (NH4)2SO4 of several vertebrate species (man, swine, buzzard, duck, goose, kestrel, kite, three tortoise species, frog, carp and perch); or in the bile of duck, goose, two snake species and frog. Only pooled chicken normal serum contained this biliary Ig type. Accordingly, the chicken biliary Ig can no longer be considered a precursor of mammalian IgA. This Ig of chicken bile represents a unique Ig class of birds and is to be called IgB (dominant biliary Ig of some birds).
利用一系列双抗体和固相放射免疫分析系统，采用兔和鲤鱼产生的重链特异性抗鸡胆汁免疫球蛋白血清以及α链特异性抗人IgA血清，对鸡胆汁免疫球蛋白（即所谓的鸡IgA）与人、猪以及鼠的IgA之间的抗原关系进行了比较研究。结果表明，鸡胆汁免疫球蛋白与IgA类免疫球蛋白之间不存在显著的免疫交叉反应。此外，在几种脊椎动物（人、猪、秃鹫、鸭、鹅、红隼、鸢、三种龟类、青蛙、鲤鱼和鲈鱼）的混合正常血清或经硫酸铵沉淀的粗免疫球蛋白组分中，以及在鸭、鹅、两种蛇类和青蛙的胆汁中，均未发现与这种鸡胆汁免疫球蛋白类似的免疫球蛋白类型。只有鸡的混合正常血清中含有这种胆汁免疫球蛋白类型。因此，鸡胆汁免疫球蛋白不能再被视为哺乳动物IgA的前体。这种鸡胆汁免疫球蛋白代表了鸟类独特的免疫球蛋白类别，应被称为IgB（某些鸟类的主要胆汁免疫球蛋白）。
