Sexton T J
US Department of Agriculture, Avian Physiology Laboratory, Beltsville, Maryland 20705.
Poult Sci. 1987 Oct;66(10):1721-6. doi: 10.3382/ps.0661721.
Three experiments were conducted to determine the effects of semen dilution, semen extender, and age of tom on the fertility of unstored semen and semen stored for 18 h at 5 C. In Experiment 1, semen was diluted 2:1, 1:1, and 1:2 with Beltsville poultry semen extender (BPSE) #2. In Experiment 2, semen was diluted 1:1 with either Lake's extender, BPSE #2, or BPSE #1. In Experiment 3, the fertility of semen from males 30 to 45-wk-old (first reproductive cycle) was compared to semen from males 51 to 66-wk-old (second reproductive cycle). Identical experiments were conducted in the fall-winter (F-W) and in the spring-summer (S-S). All hens were inseminated with 250 million spermatozoa per dose three times prior to egg production, then weekly for 15 wk. Egg fertility was determined after 7 days of incubation. In all experiments there was a significant interaction between holding time and treatment (semen dilution ratio, semen extender, or age of tom). Fertility of stored semen diluted 1:2 in Experiment 1 was lower than stored semen diluted 2:1 or 1:1 regardless of time of year (season) the experiment was conducted. Fertility was affected by a significant interaction between season and semen dilution ratio. Fertility of semen from males in F-W was higher than semen from males in S-S (Experiment 1). Semen fertility was unaffected by season when comparisons were made between semen extenders (Experiment 2) or age of tom (Experiment 3). Fertility was affected by a significant interaction between season x holding time x age of tom.
进行了三项实验，以确定精液稀释、稀释液以及公火鸡年龄对未储存精液和在5℃下储存18小时的精液受精率的影响。在实验1中，精液用贝尔茨维尔家禽精液稀释液（BPSE）#2按2:1、1:1和1:2进行稀释。在实验2中，精液用莱克稀释液、BPSE #2或BPSE #1按1:1进行稀释。在实验3中，比较了30至45周龄（第一个繁殖周期）雄性的精液与51至66周龄（第二个繁殖周期）雄性的精液的受精率。在秋冬（F-W）和春夏（S-S）进行了相同的实验。所有母鸡在产蛋前每剂量用2.5亿个精子输精三次，然后每周输精一次，持续15周。孵化7天后测定蛋的受精率。在所有实验中，保存时间和处理（精液稀释比例、精液稀释液或公火鸡年龄）之间存在显著交互作用。在实验1中，无论实验在一年中的什么时间（季节）进行，按1:2稀释的储存精液的受精率都低于按2:1或1:1稀释的储存精液。受精率受季节和精液稀释比例之间显著交互作用的影响。秋冬季节雄性的精液受精率高于春夏季节雄性的精液（实验1）。当比较不同的精液稀释液（实验2）或公火鸡年龄（实验3）时，精液受精率不受季节影响。受精率受季节×保存时间×公火鸡年龄之间显著交互作用的影响。
