Weiss G M, Pysh J J
Brain Res. 1978 Oct 13;154(2):219-30. doi: 10.1016/0006-8993(78)90696-0.
The late postnatal development of the Purkinje cell dendritic tree in mouse cerebellar vermis was investigated in Golgi preparations by morphometric techniques in order to determine at what age adult characteristics of the Purkinje cell are achieved in the rodent brain which grows continuously throughout adult life. B6D2F1 hybrid mice were sacrificed at 9, 15, 20, 35 and 250 days of age. "Hind-brain" weights (by direct weighing) and vermis volume (determined histometrically from Golgi sections), both increased rapidly from 9 to 20 days of age and continued to increase steadily with advancing age. The growth of Purkinje dendritic field areas, determined by planimetric measurements from Golgi sections paralleled the growth curves for vermis cross-sectional area, vermis volume and "hindbrain" weight. However, stereological determinations revealed an unexpected disparity between the growth of the Purkinje dendritic field areas and changes in the total length of dendrites of Purkinje cells. The total dendritic branch length per Purkinje cell increased sharply up to 20 days of age but thereafter declined with advancing age. Dendritic spine counts on Purkinje cells revealed no change in the number of dendritic spines per unit length of dendrites between 20 and 250 days of age, however, since the Purkinje cell total branch length declined-calculations suggest that the total number of spines per cell declined after 20 days of age. Thus, the size of the cerebellum and the Purkinje cell dendritic tree continued to enlarge during late postnatal development; however, the total dendritic surface area and the total number of dendritic spines on each Purkinje cell, after reaching a peak at 20 days of age, declined with advancing age. The data suggest that the late postnatal development of the Purkinje cell dendritic tree is characterized by resorption as well as dendritic growth. The functional significance of such developmental remodelling is unknown.
为了确定在整个成年期持续生长的啮齿动物大脑中，浦肯野细胞何时达到成年特征，我们通过形态测量技术在高尔基染色标本中研究了小鼠小脑蚓部浦肯野细胞树突的产后晚期发育。在9、15、20、35和250日龄时处死B6D2F1杂交小鼠。“后脑”重量（直接称重）和蚓部体积（通过对高尔基切片进行组织测量确定）在9至20日龄时均迅速增加，并随着年龄的增长持续稳定增加。通过对高尔基切片进行平面测量确定的浦肯野树突野面积的增长与蚓部横截面积、蚓部体积和“后脑”重量的生长曲线平行。然而，体视学测定显示浦肯野树突野面积的增长与浦肯野细胞树突总长度的变化之间存在意外差异。每个浦肯野细胞的树突总分支长度在20日龄前急剧增加，但此后随着年龄的增长而下降。浦肯野细胞上的树突棘计数显示，在20至250日龄之间，每单位长度树突上的树突棘数量没有变化，然而，由于浦肯野细胞总分支长度下降，计算表明每个细胞的棘突总数在20日龄后下降。因此，在产后晚期发育过程中，小脑和浦肯野细胞树突继续增大；然而，每个浦肯野细胞的树突总表面积和树突棘总数在20日龄达到峰值后随着年龄的增长而下降。数据表明，浦肯野细胞树突的产后晚期发育的特征是吸收以及树突生长。这种发育重塑的功能意义尚不清楚。
