Patel G B, Roth L A
Can J Microbiol. 1978 Aug;24(8):1007-10. doi: 10.1139/m78-166.
Two microorganisms originally existing as a mixed culture obtained from an anaerobic digester fluid were separated for pure and coculture studies. One of these was motile, Gram-negative, and non-sporeforming, and it required yeast extract for growth and acetic acid production. This isolate produced H2 and did not need H2 and (or) CO2 for growth and acetate formation. The other isolate was a methanogen whick resembled Methanobacterium arbophilicum in morphology and substrate specificity. Coculture growth of the two isolates in yeast extract broth (80% N2--20% CO2 gas phase) indicated that the non-methanogen produced up to four to five times more H2 than when grown separately. Although the growth of the non-methanogen was not enhanced by the removal of H2 by the methanogen, the hydrogen produced was essential for the growth of methanogen. Similar results were obtained when the non-methanogen was cocultured with Methanospirillum hungatti GP1. Cultivation of the non-methanogen in the presence of M. hungatti GP1 (under abundance of 80% H2--20% CO2) indicated that the acetate produced was consumed by M. hungatii, without inhibiting the growth of the other culture.
从厌氧消化池液体中获得的两种原本以混合培养物形式存在的微生物被分离出来，用于纯培养和共培养研究。其中一种微生物具有运动性，革兰氏阴性，不形成芽孢，生长和产生乙酸需要酵母提取物。该分离物产生氢气，生长和形成乙酸盐不需要氢气和（或）二氧化碳。另一种分离物是产甲烷菌，其形态和底物特异性与嗜树栖甲烷杆菌相似。在酵母提取物肉汤（80%N₂ - 20%CO₂气相）中两种分离物的共培养生长表明，非产甲烷菌产生的氢气比单独培养时多四到五倍。尽管产甲烷菌去除氢气并没有促进非产甲烷菌的生长，但产生的氢气对产甲烷菌的生长至关重要。当非产甲烷菌与Hungatii甲烷螺菌GP1共培养时也获得了类似结果。在Hungatii甲烷螺菌GP1存在的情况下（在80%H₂ - 20%CO₂充足条件下）培养非产甲烷菌表明，产生的乙酸盐被Hungatii甲烷螺菌消耗，而不抑制另一种培养物的生长。
