牛血清白蛋白降低离体大鼠肝细胞对4-甲基-2-氧代戊酸的利用。
Bovine serum albumin decreases 4-methyl-2-oxovalerate utilization by isolated rat hepatocytes.
作者信息
Livesey G
出版信息
Biochem J. 1983 Jun 15;212(3):655-8. doi: 10.1042/bj2120655.
Abstract
Binding of 4-methyl-2-oxo[1-14C]valerate to defatted bovine serum albumin inhibited the utilization of this 2-oxo acid by fed-rat hepatocytes in vitro. With 0-50g of albumin/l in the presence of 0.05mM 2-oxo acid or on increasing the 2-oxo acid concentration from 0 to 2mM in the presence of 26g of albumin/l, the extent of inhibition was essentially dependent on the change in the free 2-oxo acid concentration. Intrahepatocyte 4-methyl-2-oxo[1-14C]valerate concentrations were similar to extracellular free 2-oxo acid concentrations, suggesting equilibration so that the plasma membrane appears not to be rate-limiting for the utilization of this substrate by the isolated liver cells.
摘要
4-甲基-2-氧代[1-¹⁴C]戊酸与脱脂牛血清白蛋白的结合抑制了喂食大鼠肝细胞在体外对这种氧代酸的利用。在0.05mM氧代酸存在下白蛋白浓度为0 - 50g/L时，或者在白蛋白浓度为26g/L时将氧代酸浓度从0增加到2mM，抑制程度基本上取决于游离氧代酸浓度的变化。肝内4-甲基-2-氧代[1-¹⁴C]戊酸浓度与细胞外游离氧代酸浓度相似，表明达到平衡，因此质膜似乎不是分离的肝细胞利用该底物的限速因素。
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