Brokaw C J, Luck D J
Cell Motil. 1983;3(2):131-50. doi: 10.1002/cm.970030204.
Using a uniflagellate mutant of Chlamydomonas and flash photomicrography at 300 Hz, we have obtained detailed information on the forward and reverse beating modes of Chlamydomonas flagella and on the relationship between rotation of the uniflagellate cell and the bending cycle of the forward mode. Flagella ranging in length from 5 to 15.5 micron were photographed. There is a decrease in wavelength and an increase in curvature in the principal bends when the length of the flagellum is less than the normal length of 12-13 micron, but these changes are not sufficient to maintain similarity of the bending pattern. In the reverse mode, the flagellum propagates symmetrical, planar, undulatory waves with a shear amplitude which is the same as in the forward mode; there is a 19% increase in beat frequency and a similar decrease in wave length. The reorientation of the flagellar beat direction towards the axis of the cell in the reverse mode is caused both by the decrease in asymmetry of beat and by activation of sliding in the principal bends at an earlier time in the beat cycle, relative to the time of activation of sliding in reverse bends. There are additional rare modes of beating which may be related to intermediate stages in the transition between forward and reverse beating modes.
利用衣藻的单鞭毛突变体并以300赫兹的频率进行闪光显微摄影，我们获得了关于衣藻鞭毛正向和反向摆动模式以及单鞭毛细胞旋转与正向模式弯曲周期之间关系的详细信息。拍摄了长度在5至15.5微米之间的鞭毛。当鞭毛长度小于正常长度12 - 13微米时，主弯曲处的波长减小且曲率增加，但这些变化不足以维持弯曲模式的相似性。在反向模式中，鞭毛传播对称的平面波动波，其剪切幅度与正向模式相同；拍频增加19%，波长有类似程度的减小。反向模式中鞭毛摆动方向朝向细胞轴线的重新定向，是由摆动不对称性的降低以及相对于反向弯曲中滑动激活时间，主弯曲中滑动在摆动周期更早时间被激活所导致的。还有其他罕见的摆动模式，可能与正向和反向摆动模式转变的中间阶段有关。
