Segerbäck D
Chem Biol Interact. 1983 Jul 15;45(2):139-51. doi: 10.1016/0009-2797(83)90064-9.
Exposure of mice to 14C-labelled ethene or ethene oxide gave rise to hydroxyethylations of nucleophilic sites in hemoglobin and DNA. The relative amounts of alkylation products of different amino acids in hemoglobin were the same for the two compounds. Furthermore, the ratio between the degree of alkylation of DNA in various organs and of hemoglobin was approximately the same, supporting a previous conclusion that ethene oxide is the reactive intermediate formed in vivo from ethene. A comparison of the degrees of alkylation obtained per unit exposure dose (ppm X h) of ethene oxide and of ethene, respectively, showed that at low levels of ethene about 8% of the inhaled amount is metabolized to ethene oxide. It was estimated that the maximal rate of metabolism (Vmax) of ethene corresponds to exposure to an air level of 4 ppm of ethene oxide.
将小鼠暴露于14C标记的乙烯或环氧乙烷中，会导致血红蛋白和DNA中的亲核位点发生羟乙基化。两种化合物在血红蛋白中不同氨基酸的烷基化产物相对量相同。此外，不同器官中DNA的烷基化程度与血红蛋白的烷基化程度之比大致相同，这支持了之前的结论，即环氧乙烷是乙烯在体内形成的活性中间体。分别比较环氧乙烷和乙烯每单位暴露剂量（ppm×小时）所获得的烷基化程度，结果表明，在低水平乙烯情况下，约8%的吸入量会代谢为环氧乙烷。据估计，乙烯的最大代谢速率（Vmax）相当于暴露于空气中4 ppm的环氧乙烷水平。
