Pavlik E J, Kenady D E, van Nagell J R, Keaton K, Hanson M B, Donaldson E S, Griffen W O, Flanigan R C
Cancer Chemother Pharmacol. 1983;11(1):8-15. doi: 10.1007/BF00257408.
The physical properties of 59 anticancer agents have been examined with respect to solubility in tissue culture media, binding to ultrafiltration materials, and molecular absorbance and fluorescence behavior. Methods for dissolving these agents, which are compatible with in vitro sensitivity testing of human tumor cells to anticancer agents, are reported in this paper. The potential for anticancer agent binding to cellulose nitrate/cellulose acetate and teflon membrane ultrafilters was documented, and quantitation of these anticancer agents based upon absorbance and fluorescence spectroscopy was performed. Post-filtration quantitation of anticancer agents was found to be a reliable method for determining the actual drug concentrations available in tumor cell sensitivity testing in vitro. The properties documented herein are pharmacologically relevant parameters related to in vitro determinations of human tumor cell sensitivity to anticancer agents.
已针对59种抗癌剂在组织培养基中的溶解度、与超滤材料的结合以及分子吸光度和荧光行为对其物理性质进行了研究。本文报道了与人类肿瘤细胞对抗癌剂的体外敏感性测试兼容的这些试剂的溶解方法。记录了抗癌剂与硝酸纤维素/醋酸纤维素和聚四氟乙烯膜超滤器结合的可能性，并基于吸光光谱和荧光光谱对这些抗癌剂进行了定量。发现过滤后对抗癌剂进行定量是确定体外肿瘤细胞敏感性测试中可用实际药物浓度的可靠方法。本文记录的性质是与人类肿瘤细胞对抗癌剂的体外测定相关的药理学相关参数。
