Levinson S S, Goldman J
Clin Chim Acta. 1983 Jul 15;131(3):225-32. doi: 10.1016/0009-8981(83)90091-8.
We examined heat-treated and untreated samples for immune complexes (IC) with a nephelometric type Clq binding assay. Curves of absorbance vs. concentration derived from aggregated gamma-globulin in serum samples showed curvilinear behavior, while curves obtained from aqueous samples were highly linear. Curves from heat-treated sera bent upward, while those from unheated sera tended to level off, causing the curves to cross. Heated sera showed greater recovery of IC in the high range and unheated sera greater recovery of IC in the low. Recovery from serum-based samples varied as the concentrations of both Clq and IC in the reaction mixture changed when aqueous curves were used as calibrators. This behavior in serum-based samples seems to be caused by endogenous binders of IC, and may be an important reason for inter-laboratory variation in results from similar samples.
我们采用比浊法Clq结合试验检测了热处理和未处理样本中的免疫复合物（IC）。血清样本中聚集的γ-球蛋白得出的吸光度与浓度曲线呈曲线行为，而水性样本得到的曲线呈高度线性。热处理血清的曲线向上弯曲，而未加热血清的曲线趋于平稳，导致曲线交叉。加热血清在高浓度范围内IC回收率更高，未加热血清在低浓度范围内IC回收率更高。当用水性曲线作为校准物时，基于血清的样本回收率会随着反应混合物中Clq和IC浓度的变化而变化。基于血清的样本中的这种行为似乎是由IC的内源性结合物引起的，可能是类似样本实验室间结果差异的一个重要原因。
