Edwards S W, Hallett M B, Lloyd D, Campbell A K
FEBS Lett. 1983 Sep 5;161(1):60-4. doi: 10.1016/0014-5793(83)80730-3.
The respiratory burst of polymorphonuclear leukocytes, induced by the addition of chemotactic peptide (N-formyl-methionyl-leucyl-phenylalanine) and cytochalasin B was found to consist of two phases. The first phase of very rapid oxygen uptake lasted 1-3 min. and was followed by a second more prolonged phase of lower magnitude. The apparent Km for oxygen of unstimulated cells was 9.6 +/- 0.67 microM, while that of the second phase of stimulation was 3.7 +/- 1.6 microM oxygen. The possibility that lowered oxygen concentrations may regulate polymorphonuclear leukocyte activity in some pathological conditions is discussed.
发现添加趋化肽（N-甲酰甲硫氨酰亮氨酰苯丙氨酸）和细胞松弛素B诱导的多形核白细胞呼吸爆发由两个阶段组成。第一个非常快速的氧摄取阶段持续1 - 3分钟，随后是第二个持续时间更长、幅度更低的阶段。未刺激细胞的氧表观米氏常数为9.6±0.67微摩尔，而刺激第二阶段的氧表观米氏常数为3.7±1.6微摩尔。讨论了在某些病理条件下降低的氧浓度可能调节多形核白细胞活性的可能性。
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