用于分离和增殖街狂犬病毒的臭鼬原代脑和肾以及浣熊肾细胞与已建立细胞系的比较。
Comparison of primary skunk brain and kidney and raccoon kidney cells with established cell lines for isolation and propagation of street rabies virus.
作者信息
Umoh J U, Blenden D C
出版信息
Infect Immun. 1983 Sep;41(3):1370-2. doi: 10.1128/iai.41.3.1370-1372.1983.
Abstract
Cell cultures prepared from skunk kidney, raccoon kidney, and skunk brain were compared with CER, murine neuroblastoma (C1300, clone NA), baby hamster kidney (BHK-21, S-13), and dog kidney (MDCK) cell lines for virus isolation and propagation of street and fixed rabies virus. The skunk brain cells were suitable for efficient replication of all the virus isolates. They were comparable to CER and murine neuroblastoma cells for virus isolation and propagation. None of the other cell cultures was satisfactory. Further work is under way to refine the skunk brain cell cultures.
摘要
将从臭鼬肾脏、浣熊肾脏和臭鼬大脑制备的细胞培养物与CER、鼠神经母细胞瘤（C1300，克隆NA）、幼仓鼠肾（BHK - 21，S - 13）和狗肾（MDCK）细胞系进行比较，以分离病毒并繁殖街毒株和固定毒株狂犬病病毒。臭鼬脑细胞适合于所有病毒分离株的高效复制。在病毒分离和繁殖方面，它们与CER和鼠神经母细胞瘤细胞相当。其他细胞培养物均不理想。目前正在进一步开展工作以优化臭鼬脑细胞培养物。
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