Xu C X, Hendry J H, Testa N G, Allen T D
J Cell Sci. 1983 May;61:453-66. doi: 10.1242/jcs.61.1.453.
Modifications to the colony assay in vitro for stromal progenitor cells in mouse femoral marrow have been studied so as to optimize the efficiency of colony formation (CFE). The highest reproducible concentration achieved was about 30 colonies (containing fibroblasts, macrophages and endothelioid cells) per 10(6) nucleated marrow cells (range 20-50) in mice 3-4 months old, and higher by 50% in mice 14-15 months old. Each of many slight technical modifications could reduce these values by more than 30%. The importance of optimization was demonstrated by a reduced radiosensitivity when the CFE was reduced by a factor of 3 using alpha-medium stored at 4 degrees C for 15 days. The D0 value was 3.9 +/- 0.8 Gy compared to 1.6 +/- 0.1 Gy using freshly prepared medium, and this could be due to the selection of a radioresistant subpopulation. The modifications studied may partly explain the marked variations in CFE and in radiosensitivity reported in the literature.
为优化集落形成效率（CFE），对小鼠股骨骨髓基质祖细胞的体外集落测定方法进行了研究。在3至4月龄小鼠中，每10⁶ 个有核骨髓细胞（范围为20 - 50个）可实现的最高可重复性集落浓度约为30个集落（包含成纤维细胞、巨噬细胞和内皮样细胞），而在14至15月龄小鼠中则高出50%。许多微小的技术改进中的每一项都可能使这些数值降低超过30%。当使用在4℃储存15天的α - 培养基使CFE降低3倍时，放射敏感性降低，这证明了优化的重要性。与使用新鲜制备的培养基时的1.6 ± 0.1 Gy相比，D0值为3.9 ± 0.8 Gy，这可能是由于选择了放射抗性亚群。所研究的改进可能部分解释了文献中报道的CFE和放射敏感性的显著差异。
