鉴定肺炎球菌附着于人咽上皮细胞的糖缀合物受体的活性二糖单元。
Identification of an active disaccharide unit of a glycoconjugate receptor for pneumococci attaching to human pharyngeal epithelial cells.
作者信息
Andersson B, Dahmén J, Frejd T, Leffler H, Magnusson G, Noori G, Edén C S
出版信息
J Exp Med. 1983 Aug 1;158(2):559-70. doi: 10.1084/jem.158.2.559.
Abstract
Glycoconjugates containing the disaccharide unit GlcNAc beta 1 leads to 3Gal beta were suggested as receptors for pneumococci adhering to human pharyngeal epithelial cells. The receptor activity was detected both by inhibition of adhesion by an excess of free oligosaccharide and by induction or increase of adhesion after coating of target cells with glycolipid. Studies with free natural and synthetic oligosaccharides identified the disaccharide GlcNAc beta 1 leads to 3Gal beta as one critical binding site. The specificity of recognition was shown inter alia by the lack of inhibitory activity of GlcNAc beta 1 leads to 4Gal beta, which differs only in the linkage of the two sugars. Specific interference with pneumococcal adhesion by administration of soluble receptor sugar may improve our understanding of the role of adhesion in vivo.
摘要
含有二糖单位β-1,3连接的N-乙酰葡糖胺（GlcNAc）和β-半乳糖（Gal）的糖缀合物被认为是肺炎球菌黏附人咽上皮细胞的受体。通过过量游离寡糖抑制黏附以及用糖脂包被靶细胞后诱导或增加黏附，均可检测到受体活性。对游离天然和合成寡糖的研究确定二糖β-1,3连接的N-乙酰葡糖胺和β-半乳糖是一个关键结合位点。尤其是β-1,4连接的N-乙酰葡糖胺和β-半乳糖缺乏抑制活性，这表明识别具有特异性，二者仅在两种糖的连接方式上有所不同。通过给予可溶性受体糖特异性干扰肺炎球菌黏附，可能会增进我们对黏附在体内作用的理解。
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本文引用的文献
1
Selective bacterial adherence to oral epithelial surfaces and its role as an ecological determinant.选择性细菌黏附于口腔上皮表面及其作为生态决定因素的作用。
Infect Immun. 1971 Apr;3(4):567-73. doi: 10.1128/iai.3.4.567-573.1971.
2
BLOOD GROUP ACTIVE GLYCOLIPID FROM HUMAN ERYTHROCYTES.来自人类红细胞的血型活性糖脂。
Jpn J Exp Med. 1963 Dec;33:347-60.
3
An epidemiologic approach to pneumococcal disease.肺炎球菌疾病的流行病学研究方法。
Rev Infect Dis. 1981 Mar-Apr;3(2):233-45. doi: 10.1093/clinids/3.2.233.
4
Inhibition of experimental ascending urinary tract infection by an epithelial cell-surface receptor analogue.上皮细胞表面受体类似物对实验性上行性尿路感染的抑制作用
Nature. 1982 Aug 5;298(5874):560-2. doi: 10.1038/298560a0.
5
Glycolipid receptors for uropathogenic Escherichia coli on human erythrocytes and uroepithelial cells.人红细胞和尿道上皮细胞上尿路致病性大肠杆菌的糖脂受体
Infect Immun. 1981 Dec;34(3):920-9. doi: 10.1128/iai.34.3.920-929.1981.
6
Glycosphingolipids in cellular interaction, differentiation, and oncogenesis.细胞相互作用、分化及肿瘤发生过程中的糖鞘脂
Annu Rev Biochem. 1981;50:733-64. doi: 10.1146/annurev.bi.50.070181.003505.
7
The role of complement in the host's defense against Streptococcus pneumoniae.补体在宿主抵御肺炎链球菌中的作用。
Rev Infect Dis. 1981 Mar-Apr;3(2):289-98. doi: 10.1093/clinids/3.2.289.
8
The epidemiology of pneumococcal disease in infants and children.婴幼儿肺炎球菌疾病的流行病学
Rev Infect Dis. 1981 Mar-Apr;3(2):246-53. doi: 10.1093/clinids/3.2.246.
9
Growth characteristics of group A streptococci in a new chemically defined medium.A组链球菌在一种新的化学成分明确的培养基中的生长特性。
Infect Immun. 1980 Feb;27(2):444-8. doi: 10.1128/iai.27.2.444-448.1980.
10
Fibronectin-mediated binding of group A streptococci to human polymorphonuclear leukocytes.纤连蛋白介导A组链球菌与人类多形核白细胞的结合。
Infect Immun. 1982 Aug;37(2):805-10. doi: 10.1128/iai.37.2.805-810.1982.
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