Kattermann R, Kupke I R, Borner K, Assmann G, Eggstein M, Gambert P, Heuck C C, Kreutz F H, Seidel D, Siekmann L, Stamm D, Wieland H
J Clin Chem Clin Biochem. 1983 Jun;21(6):347-55.
An enzymatic method is described for the determination of total cholesterol in serum using a single aqueous reagent which can easily be prepared from commercial substrates and enzymes. The determination is carried out manually, the resulting stable chromogen is measured at a wavelength of 500-550 nm. The cholesterol concentration may be calculated either using a primary cholesterol standard or a constant factor for a given wavelength. The reliability of the method is reported: Data for the imprecision are given on the basis of a survey in 3 laboratories, the accuracy is established by comparison with a definitive and a reference method. Analytical and biological interferances are briefly discussed and results with this enzymatic method are reported concerning the reference values for serum cholesterol.
