Toowicharanont P, Chulavatnatol M
J Reprod Fertil. 1983 Sep;69(1):303-6. doi: 10.1530/jrf.0.0690303.
Anionic sites of rat epididymal spermatozoa were measured at pH 7.4 using tritiated polycationized ferritin. The spermatozoa from the caput region had 1.25 +/- 0.06 X 10(6) anionic sites per cell and a binding constant of 1.26 +/- 0.01 X 10(6) M-1. Spermatozoa from the cauda region had 1.50 +/- 0.09 X 10(6) anionic sites per cell and a binding constant of 4.84 +/- 0.82 X 10(6) M-1. The values were mean +/- s.d. The anionic sites were partly sensitive to treatments with neuraminidase, trypsin and Triton X-100.
使用氚标记的聚阳离子铁蛋白在pH 7.4条件下测定大鼠附睾精子的阴离子位点。来自附睾头区域的精子每个细胞有1.25±0.06×10⁶个阴离子位点，结合常数为1.26±0.01×10⁶ M⁻¹。来自附睾尾区域的精子每个细胞有1.50±0.09×10⁶个阴离子位点，结合常数为4.84±0.82×10⁶ M⁻¹。这些值为平均值±标准差。阴离子位点对神经氨酸酶、胰蛋白酶和曲拉通X-100处理部分敏感。
