Ruf K B
J Steroid Biochem. 1983 Jul;19(1C):887-90. doi: 10.1016/0022-4731(83)90029-8.
The biological effects of exogenous 5 alpha-androstane-3 alpha,17 beta-diol (3 alpha-diol) and its 3 beta-epimer (3 beta-diol) on sexual maturation were assessed in Sprague-Dawley rats. Chronic administration of 3 alpha-diol, but not of 3 beta-diol, prevented ovulation and suppressed uterine and ovarian development. Vaginal opening was advanced by 3 alpha-diol, but neither vaginal cytology nor ovarian histology revealed any signs of ovarian cyclicity. When administered acutely, 3 alpha-diol prevented the estrogen-priming required for the triggering of gonadotropin surges by progesterone as well as the estrogen-sensitization of pituitary gonadotrophs to LHRH stimulation. Plasma concentrations of endogenous free 3 alpha- and 3 beta-diol and of testosterone and dihydrotestosterone were assessed during spontaneous and PMSG-induced sexual maturation. The observations are compatible with the postulate of a puberty-related shift in ovarian biosynthetic pathways from the preferential 5 alpha-reduction of progesterone to the production of aromatizable androgens.
在斯普拉格-道利大鼠中评估了外源性5α-雄甾烷-3α,17β-二醇（3α-二醇）及其3β-差向异构体（3β-二醇）对性成熟的生物学效应。长期给予3α-二醇可阻止排卵并抑制子宫和卵巢发育，但给予3β-二醇则无此作用。3α-二醇可使阴道开口提前，但阴道细胞学检查和卵巢组织学检查均未发现卵巢周期性变化的任何迹象。急性给予3α-二醇可阻止孕酮引发促性腺激素激增所需的雌激素预处理，以及垂体促性腺细胞对促黄体生成素释放激素（LHRH）刺激的雌激素敏感性。在自发和孕马血清促性腺激素（PMSG）诱导的性成熟过程中，评估了内源性游离3α-二醇、3β-二醇、睾酮和双氢睾酮的血浆浓度。这些观察结果与以下假设相符：即青春期相关的卵巢生物合成途径从优先将孕酮5α-还原转变为产生可芳香化的雄激素。
