Tyutyulkova N, Gorantcheva U, Tuneva S, Chelibonova-Lorer H, Gavasova E, Zhivkov V
Methods Find Exp Clin Pharmacol. 1983;5(3):181-4.
The effect of the hepatoprotective drug silymarin (Carsil) on the incorporation rate of 14C-leucine into total proteins and on the biosynthesis of UDP-N-acetylhexosamine and microsomal glycoproteins using 14C-glucose of rat liver with D-galactosamine hepatitis was studied. It was found that i.p. treatment with Carsil in a dose of 140 mg/kg applied for 4 consecutive days partly abolishes the inhibitory effect of galactosamine on protein and glycoprotein biosynthesis. The specific activity of 14C-labelled UDP-N-acetylhexosamine is higher in the liver of D-galactosamine treated rats, compared with the specific activity of the nucleotide from liver pretreated with Carsil and then injected with galactosamine. This fact supports the suggestion that Carsil probably activates the metabolic conversion of UDP-hexosamine to the acetylated metabolite-UDP-N-acetylhexosamine, which is the normal liver cell metabolite, in liver cells.
研究了保肝药物水飞蓟素（利加隆）对用14C-亮氨酸掺入大鼠肝脏总蛋白的掺入率以及对用14C-葡萄糖合成UDP-N-乙酰己糖胺和微粒体糖蛋白的生物合成的影响，该大鼠肝脏患有D-半乳糖胺肝炎。结果发现，连续4天腹腔注射140mg/kg剂量的利加隆可部分消除半乳糖胺对蛋白质和糖蛋白生物合成的抑制作用。与先用利加隆预处理然后注射半乳糖胺的大鼠肝脏中核苷酸的比活性相比，D-半乳糖胺处理的大鼠肝脏中14C标记的UDP-N-乙酰己糖胺的比活性更高。这一事实支持了以下观点：利加隆可能激活了UDP-己糖胺在肝细胞中向正常肝细胞代谢物——乙酰化代谢物UDP-N-乙酰己糖胺的代谢转化。
