组织型纤溶酶原激活物的简易定量测定法。
A simple quantitative assay of tissue plasminogen activator.
作者信息
De Cossart L, Marcuson R W
出版信息
J Clin Pathol. 1982 Sep;35(9):980-3. doi: 10.1136/jcp.35.9.980.
A simple method for the quantitative assay of tissue plasminogen activator is described. Human veins and uterus obtained at operation are disintegrated in a membrane disintegrator at -70 degrees C and a known weight of the powder, suspended in buffered saline and thoroughly mixed. Assay of the dilutions of this homogenate on isotope-labelled fibrin clots gives straight line plots of log weight against log activity of the dilution and the sample activity is calculated from this. The method has been compared with the conventional histochemical technique. A highly significant correlation between the results of the two methods has been obtained (r = 0.79; p less than 0.001).
本文描述了一种组织纤溶酶原激活剂定量测定的简单方法。手术中获取的人静脉和子宫在-70℃的膜式匀浆器中匀浆，取已知重量的粉末悬浮于缓冲盐溶液中并充分混合。用该匀浆的稀释液检测同位素标记的纤维蛋白凝块，以稀释液的对数重量对对数活性作图可得直线，据此计算样品活性。该方法已与传统组织化学技术进行比较。两种方法的结果之间存在高度显著的相关性（r = 0.79；p < 0.001）。
Zotero 插件