基于分离大豆蛋白的饮食中锌的生物利用度：稳定同位素示踪剂70Zn在年轻男性中的应用。

Bioavailability of zinc from a diet based on isolated soy protein: application in young men of the stable isotope tracer, 70Zn.

作者信息

Solomons N W, Janghorbani M, Ting B T, Steinke F H, Christensen M, Bijlani R, Istfan N, Young V R

出版信息

J Nutr. 1982 Oct;112(10):1809-21. doi: 10.1093/jn/112.10.1809.

DOI:10.1093/jn/112.10.1809

Abstract

With the aid of the stable isotope, 70Zn, as a tracer and neutron activation analysis, a combination of extrinsic labeling of meals and fecal monitoring of isotope excretion was used as a safe and noninvasive approach for assessing the effects of the vegetable (soy) and animal (milk, beef) proteins on the absorption of zinc in healthy, adult human volunteers. A known amount of 70Zn was added as ZnCl2, to six consecutive meals over a 2-day period during which either one of three isonitrogenous liquid formulas (skim milk; soy isolate; or a 50:50 mixture) or one of two bologna sausages (soy isolate of beef) were given. The mean absorption of 70Zn from milk, soy and soy/milk was 41 +/- 4, 34 +/- 4, and 41 +/- 7% (mean +/- SEM), respectively, the presence of soy protein having no effect on absorption of the extrinsic label. For beef bologna and soy bologna, fractional absorption of the 70Zn tracer was 41 +/- 4 and 30 +/- 3%, respectively. Beef might favor absorption of extrinsic zinc. The kinetics of isotope excretion, pooling procedures, for stool samples and the utility of fecal markers were also evaluated.

摘要

借助稳定同位素70Zn作为示踪剂并采用中子活化分析，将膳食的外源性标记与粪便中同位素排泄监测相结合，作为一种安全、无创的方法，用于评估蔬菜（大豆）和动物（牛奶、牛肉）蛋白对健康成年志愿者锌吸收的影响。在为期2天的时间里，将已知量的70Zn以ZnCl2的形式添加到连续六餐中，在此期间，分别给予三种等氮液体配方（脱脂牛奶；大豆分离蛋白；或50:50混合物）之一，或两种博洛尼亚香肠（牛肉大豆分离蛋白）之一。牛奶、大豆和大豆/牛奶中70Zn的平均吸收率分别为41±4%、34±4%和41±7%（平均值±标准误），大豆蛋白的存在对外源性标记物的吸收没有影响。牛肉博洛尼亚香肠和大豆博洛尼亚香肠中70Zn示踪剂的吸收率分别为41±4%和30±3%。牛肉可能有利于外源性锌的吸收。还评估了粪便样品的同位素排泄动力学、合并程序以及粪便标记物的效用。