March W F, Rabinovitch B, Adams R L
Diabetes Care. 1982 May-Jun;5(3):259-65. doi: 10.2337/diacare.5.3.259.
We have discussed the nature of a scleral lens that will allow us to follow changes in aqueous humor glucose levels in animals by a method based on optical rotation and a technique described in an earlier paper. We have shown how this lens can be micro-miniaturized and can be used in humans as a non-invasive glucose monitor. We have described preliminary experiments designed to show the correlation between the blood glucose assay (BGA) and the aqueous humor glucose concentration as determined by chemical assay (AGA) and by optical rotation determination (ARD). The last mentioned has been obtained by paracentesis directly into a microcell used in conjunction with instrumentation capable of measuring optical rotations as low as 0.0013 degrees (4.5") corresponding to 20 mg/dl glucose with a sensitivity of 0.0001 degrees (0.36"). The variability among normal rabbits as a function of individuality and diurnal changes is described, and the correlation between AGA and ARD shown to be essentially 1.0. Such rabbits are examined when undergoing very rapid decreases in BGA (insulin treatment) or very rapid increases in BGA (bolus of glucose). The AGA and ARD are shown to lag behind the BGA, and this is discussed in terms of the rate of change of BGA with respect to time and its concomitant change in AGA/ARD as well as a simple procedure that would materially reduce this lag.
我们已经讨论了一种巩膜透镜的特性，该透镜将使我们能够通过基于旋光性的方法以及早期论文中描述的技术来跟踪动物房水葡萄糖水平的变化。我们已经展示了这种透镜如何能够被微小型化，并可作为一种非侵入性葡萄糖监测器用于人类。我们描述了旨在显示血糖测定（BGA）与通过化学测定（AGA）和旋光测定（ARD）所确定的房水葡萄糖浓度之间相关性的初步实验。后者是通过直接穿刺进入一个微细胞获得的，该微细胞与能够测量低至0.0013度（4.5"）旋光性的仪器一起使用，这对应于20mg/dl葡萄糖，灵敏度为0.0001度（0.36"）。描述了正常兔子个体差异和昼夜变化导致的变异性，并且显示AGA和ARD之间的相关性基本为1.0。在兔子血糖水平快速下降（胰岛素治疗）或快速上升（葡萄糖推注）时对其进行检查。结果显示AGA和ARD滞后于BGA，并根据BGA随时间的变化率及其在AGA/ARD中的伴随变化以及一种可大幅减少这种滞后的简单程序对此进行了讨论。
