Iogannsen M G, Dolgopiatova M A, Prokof'eva O G, Pliss G B
Vopr Onkol. 1980;26(1):70-2.
From the tissue of the transplantable strain of rat lymphosarcoma, using phenol technics, a total RNA (sedimentation constants 31,5S; 19,0S; 6S) and chromosome-nuclear mRNA were isolated. The preparations contained up to 10% of protein. Nucleic acids (in a dosage of 10 mg RNA) were given by single intraperitoneal injections to 2 month-old rats. The animals showed a 180% increase of the lymphatic neoplasms yield when injected both total RNA and mRNA. Transplantation into newborn rats of the rat lymphocytes, treated in vitro by the total RNA preparations, failed to yield such a significant increase in the occurrence of neoplasms. A molecular weight of RNA fragments was similar to that in the experiments on transfection by nucleic acids, isolated from tumors of viral etiology.
从可移植性大鼠淋巴肉瘤品系的组织中，运用苯酚技术分离出了总RNA（沉降常数分别为31.5S、19.0S、6S）和染色体核mRNA。这些制剂含有高达10%的蛋白质。以10毫克RNA的剂量通过单次腹腔注射给予2月龄大鼠。当同时注射总RNA和mRNA时，动物的淋巴肿瘤产量增加了180%。用总RNA制剂在体外处理过的大鼠淋巴细胞移植到新生大鼠体内，肿瘤发生率并未出现如此显著的增加。RNA片段的分子量与从病毒病因肿瘤中分离的核酸进行转染实验中的分子量相似。
