Stepan'ian L I, Vakhtin Iu B
Tsitologiia. 1980 Feb;22(2):198-204.
Cell populations of eight experimental tumors (murine and rat rhabdomyosarcomas induced with 20-methylcholantrene, transplantable murine rhabdomyosarcoma A-7, and transplantable rat lymphosarcoma) have been selected for the affinity of their cells to lung tissue. The level of the affinity was measured as the number of lung nodules per 100 000 tumor cells injected intravenously. A 3-4-fold selection appeared to be non-effective, whereas 10-fold or more prolonged selections resulted in a gradual enhancement of the affinity of tumor cells to lung tissue. Thus, the transplantable murine and rat rhabdomyosarcomas were obtained with an increased capacity of their cells of yielding lung nodules. The affinity of rat rhabdomyosarcoma was 200-300 times higher after 40 steps of selection compared to the initial tumor affinity. With the rhabdomyosarcoma of CC57W mice, the affinity increased by 5 times after 20 steps of selection. Using our technique of selection (without an in vitro cultivation), the capacity of cells of persisting in lung tissue and yielding lung nodules looks likely as a quantitative character with a rather low heritability. It has been concluded that in cell populations of tumors examined there are only a few genetic population variations in cell capacity of making non-random metastases.
已根据八种实验性肿瘤（用20-甲基胆蒽诱导的小鼠和大鼠横纹肌肉瘤、可移植的小鼠横纹肌肉瘤A-7以及可移植的大鼠淋巴肉瘤）细胞对肺组织的亲和力进行了细胞群体筛选。亲和力水平通过静脉注射每100000个肿瘤细胞所形成的肺结节数量来衡量。3至4倍的筛选似乎无效，而10倍或更高倍数的延长筛选会导致肿瘤细胞对肺组织的亲和力逐渐增强。因此，获得了可移植的小鼠和大鼠横纹肌肉瘤，其细胞产生肺结节的能力增强。经过40步筛选后，大鼠横纹肌肉瘤的亲和力比初始肿瘤亲和力高200至300倍。对于CC57W小鼠的横纹肌肉瘤，经过20步筛选后，亲和力提高了5倍。使用我们的筛选技术（无需体外培养），细胞在肺组织中持续存在并产生肺结节的能力似乎是一种遗传力相当低的数量性状。得出的结论是，在所检查的肿瘤细胞群体中，细胞产生非随机转移的能力只有少数遗传群体变异。
