胃泌酸调节素包含胰高血糖素完整序列的证据。
Evidence that glicentin contains the entire sequence of glucagon.
作者信息
Holst J J
出版信息
Biochem J. 1980 May 1;187(2):337-43. doi: 10.1042/bj1870337.
Abstract
Glicentin (a highly purified 100-amino acid peptide with glucagon-like immunoreactivity from porcine gut) was subjected to limited digestion with trypsin and carboxypeptidase B, and the resulting peptides were studied by gel filtration and region-specific glucagon radioimmunoassays. Similar digests of glucagon and purified fragments of glucagon were studied in parallel. Glicentin gave rise to peptides that corresponded closely to the 1-17 and 19-29 fragments of glucagon. Also, 125I-labelled glicentin and 125I-labelled glucagon gave rise to identical fragments after trypsin treatment. On the basis of this and other evidence [Jacobsen, Demandt, Moody & Sundby (1977) Biochim. Biophys. Acta 493, 452-459] it is concluded that glicentin contains the entire glucagon sequence at residues number 64-92 and thus fulfills one of the requirements for being a 'proglucagon'.
摘要
胰高血糖素原（一种从猪肠道中提取的具有胰高血糖素样免疫反应性的高度纯化的100个氨基酸的肽）用胰蛋白酶和羧肽酶B进行有限度消化，所得肽段通过凝胶过滤和区域特异性胰高血糖素放射免疫测定法进行研究。同时对胰高血糖素和胰高血糖素纯化片段进行类似消化并研究。胰高血糖素原产生的肽段与胰高血糖素的1-17和19-29片段密切对应。此外，经胰蛋白酶处理后，125I标记的胰高血糖素原和125I标记的胰高血糖素产生相同的片段。基于此及其他证据[雅各布森、德芒特、穆迪和桑德比（1977年）《生物化学与生物物理学学报》493卷，452 - 459页]，得出结论：胰高血糖素原在64 - 92位残基处包含完整的胰高血糖素序列，因此满足作为“胰高血糖素原”的一项要求。
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