肠高血糖素（II）与胰高血糖素原的C末端序列（第33 - 69位氨基酸残基）相同的证据。
Evidence that enteroglucagon (II) is identical with the C-terminal sequence (residues 33-69) of glicentin.
作者信息
Holst J J
出版信息
Biochem J. 1982 Dec 1;207(3):381-8. doi: 10.1042/bj2070381.
Abstract
Enteroglucagon (II) was isolated from extracts of pig ileum mucosa by repeated gel filtrations, and its immunochemical and chromatographic characteristics were compared with those of a synthetic peptide corresponding to the 33-69 sequence of pig glicentin, before and after digestion with trypsin or trypsin followed by carboxypeptidase B, by using five region-specific assays covering most of the glicentin sequence. Enteroglucagon (II) and the synthetic peptide behave identically under three different conditions of chromatography as determined with all five assays (including a highly specific radioreceptor assay), and gave rise to similar fragments after enzyme digestion. It was therefore concluded that enteroglucagon (II) and the 33-69 sequence of glicentin are most probably identical.
摘要
通过反复凝胶过滤从猪回肠黏膜提取物中分离出肠高血糖素（II），并使用涵盖大部分胰高血糖素原序列的五种区域特异性检测方法，比较了其与猪胰高血糖素原33 - 69序列对应的合成肽在胰蛋白酶消化或胰蛋白酶后接羧肽酶B消化前后的免疫化学和色谱特征。在所有五种检测方法（包括高度特异性的放射受体检测）所确定的三种不同色谱条件下，肠高血糖素（II）和合成肽表现相同，并且酶消化后产生相似的片段。因此得出结论，肠高血糖素（II）和胰高血糖素原的33 - 69序列很可能是相同的。
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