Greenwald I S, Horvitz H R
Genetics. 1980 Sep;96(1):147-64. doi: 10.1093/genetics/96.1.147.
The uncoordinated, egg-laying-defective mutation, unc-93(e1500) III, of the nematode Caenorhabditis elegans spontaneously reverts to a wild-type phenotype. We describe 102 spontaneous and mutagen-induced revertants that define three loci, two extragenic (sup-9 II and sup-10 X) and one intragenic. Genetic analysis suggests that e1500 is a rare visible allele that generates a toxic product and that intragenic reversion, resulting from the generation of null alleles of the unc-93 gene, eliminates the toxic product. We propose that the genetic properties of the unc-93 locus, including the spontaneous reversion of the e1500 mutation, indicate that unc-93 may be a member of a multigene family. The extragenic suppressors also appear to arise as the result of elimination of gene activity; these genes may encode regulatory functions or products that interact with the unc-93 gene product. Genes such as unc-93, sup-9 and sup-10 may be useful for genetic manipulations, including the generation of deficiencies and mutagen testing.
线虫秀丽隐杆线虫（Caenorhabditis elegans）的不协调、产卵缺陷突变体unc-93(e1500) III会自发回复为野生型表型。我们描述了102个自发和诱变诱导的回复体，它们定义了三个位点，两个基因外位点（sup-9 II和sup-10 X）和一个基因内位点。遗传分析表明，e1500是一个罕见的可见等位基因，会产生一种有毒产物，而由unc-93基因无效等位基因的产生导致的基因内回复消除了这种有毒产物。我们提出，unc-93位点的遗传特性，包括e1500突变的自发回复，表明unc-93可能是一个多基因家族的成员。基因外抑制子似乎也是基因活性消除的结果；这些基因可能编码与unc-93基因产物相互作用的调控功能或产物。诸如unc-93、sup-9和sup-10等基因可能有助于进行遗传操作，包括缺失的产生和诱变测试。
