Greenwald I S, Horvitz H R
Genetics. 1982 Jun;101(2):211-25. doi: 10.1093/genetics/101.2.211.
The sup-11 1 locus of C. elegans was defined by rare dominant suppressors of unc-93(e1500) III, a mutation that affects muscle structure. All ten of these dominant suppressors have a recessive "scrawny" phenotype. Two additional classes of sup-11 alleles were identified. One class, null alleles, was obtained by reversion of the dominant suppressor activity. These null alleles are recessive embryonic lethals, indicating that sup-11 is an essential gene. Members of the second class, rare semidominant revertants of the "scrawny" phenotype, are partial suppressors of unc-93(e1500). The genetic properties of the dominant suppressor mutations suggest that they are rare missense mutations that confer a novel activity to the sup-11 protein. We consider some of the ways that sup-11 alleles might suppress unc-93(e1500), including the possibilities that the altered sup-11 proteins restore function to a protein complex or are modified products of a gene that is a member of an unc-93 gene family.
秀丽隐杆线虫的sup-11 1基因座由unc-93(e1500) III的罕见显性抑制子定义,unc-93(e1500) III是一个影响肌肉结构的突变。所有这十个显性抑制子都具有隐性的“瘦骨嶙峋”表型。另外还鉴定出了两类sup-11等位基因。一类是无效等位基因,通过显性抑制子活性的回复突变获得。这些无效等位基因是隐性胚胎致死基因,表明sup-11是一个必需基因。第二类的成员,即“瘦骨嶙峋”表型的罕见半显性回复突变体,是unc-93(e1500)的部分抑制子。显性抑制子突变的遗传特性表明它们是罕见的错义突变,赋予了sup-11蛋白一种新活性。我们考虑了sup-11等位基因可能抑制unc-93(e1500)的一些方式,包括改变后的sup-11蛋白恢复蛋白复合物功能的可能性,或者是unc-93基因家族成员基因的修饰产物。