Tatsumi H, Shimada N, Kuramoto R, Mochizuki Y, Nishizima M, Arai M, Osanai K, Ishihara K, Goso K, Hotta K
Acta Obstet Gynaecol Jpn. 1981 May;33(5):643-50.
A simple and sensitive procedure for the quantitative estimation of pulmonary surfactants in the amniotic fluid is described. The method is based on the formation of turbidity from the amniotic fluid surfactants with phenol. Amniotic fluid drawn through an intrauterine catheter was centrifuged at 2000 rpm for 5 min. One ml of 5% aqueous phenol solution was added to 1.0 ml of the diluted supernatant. Control solution was prepared by addition of 1.0 ml of distilled water to the supernatant, instead of phenol solution. The turbidity was measured spectrophotometrically against control at 340 nm 5 to 10 min after agitation on a Vortex mixer for 5 sec. The turbidity obtained from amniotic fluid with phenol was proportional to the increase in total phospholipids in the fluid measured enzymatically. Moreover, only lecithin in the phospholipids was related to the turbidity formation. Sphyngomyelin, lysolecithin, phosphatidylethanolamin did not produce any turbidity with phenol. The spectrophotometric reading of 87 cases (31-41 weeks) was ranged at 0.18-3.52. Four cases with lower value (0.40) showed neonatal respiratory problem. The phenol turbidity test is more useful for the detection of fetal lung maturity comparing with the generally used shake test.
本文描述了一种简单且灵敏的定量测定羊水肺表面活性剂的方法。该方法基于羊水表面活性剂与苯酚形成浊度。通过宫内导管抽取的羊水以2000转/分钟的速度离心5分钟。将1毫升5%的苯酚水溶液加入到1.0毫升稀释后的上清液中。对照溶液的制备方法是向上清液中加入1.0毫升蒸馏水，而不是苯酚溶液。在涡旋混合器上搅拌5秒后，于340纳米处，在5至10分钟内以对照为参比，用分光光度计测量浊度。从含苯酚的羊水中获得的浊度与通过酶法测定的羊水中总磷脂的增加量成正比。此外，磷脂中只有卵磷脂与浊度形成有关。鞘磷脂、溶血卵磷脂、磷脂酰乙醇胺与苯酚不会产生任何浊度。87例（孕周31 - 41周）的分光光度读数范围为0.18 - 3.52。4例读数较低（0.40）的病例出现了新生儿呼吸问题。与常用的振荡试验相比，苯酚浊度试验在检测胎儿肺成熟度方面更有用。
