Dasgupta R, Kaesberg P
Nucleic Acids Res. 1982 Jan 22;10(2):703-13. doi: 10.1093/nar/10.2.703.
The nucleotide sequences of the subgenomic coat protein messengers (RNA4's) of two related bromoviruses, brome mosaic virus (BMV) and cowpea chlorotic mottle virus (CCMV), have been determined by direct RNA and CDNA sequencing without cloning. BMV RNA4 is 876 b long including a 5' noncoding region of nine nucleotides and a 3' noncoding region of 300 nucleotides. CCMV RNA 4 is 824 b long, including a 5' noncoding region of 10 nucleotides and a 3' noncoding region of 244 nucleotides. The encoded coat proteins are similar in length (188 amino acids for BMV and 189 amino acids for CCMV) and display about 70% homology in their amino acid sequences. Length difference between the two RNAs is due mostly to a single deletion, in CCMV with respect to BMV, of about 57 b immediately following the coding region. Allowing for this deletion the RNAs are indicate that mutations leading to divergence were constrained in the coding region primarily by the requirement of maintaining a favorable coat protein structure and in the 3' noncoding region primarily by the requirement of maintaining a favorable RNA spatial configuration.
通过直接RNA和cDNA测序（无需克隆），已确定了两种相关的雀麦花叶病毒（BMV）和豇豆褪绿斑驳病毒（CCMV）亚基因组外壳蛋白信使（RNA4）的核苷酸序列。BMV RNA4长876个碱基，包括一个9个核苷酸的5'非编码区和一个300个核苷酸的3'非编码区。CCMV RNA4长824个碱基，包括一个10个核苷酸的5'非编码区和一个244个核苷酸的3'非编码区。编码的外壳蛋白长度相似（BMV为188个氨基酸，CCMV为189个氨基酸），其氨基酸序列显示约70%的同源性。两种RNA之间的长度差异主要是由于CCMV相对于BMV在编码区之后紧接着有一个约57个碱基的单缺失。考虑到这种缺失，RNA表明导致分化的突变在编码区主要受维持有利的外壳蛋白结构的需求限制，在3'非编码区主要受维持有利的RNA空间构型的需求限制。
