Nordenson I, Sweins A, Beckman L
Scand J Work Environ Health. 1981 Dec;7(4):277-81. doi: 10.5271/sjweh.2551.
Cultured human lymphocytes were exposed to trivalent (NaASO2) and pentavalent (Na2HAsO4) arsenic in concentrations comparable to the arsenic levels found in the urine of copper smelter workers. Significantly increased frequencies of chromosome aberrations (gaps, chromatid breaks, chromatid exchanges and chromosome breaks) were found after exposure to trivalent but not pentavalent arsenic. This effect was not found when nonstimulated (GO) lymphocytes were exposed to trivalent arsenic and then cultured. The rate of sister chromatid exchanges was also found to be increased after exposure to trivalent arsenic. Thus results suggest that trivalent arsenic is more genotoxic than pentavalent arsenic and that arsenic exerts its effect mainly during cell division.
将培养的人类淋巴细胞暴露于三价砷（亚砷酸钠）和五价砷（砷酸氢二钠）中，其浓度与铜冶炼工人尿液中发现的砷水平相当。暴露于三价砷后，染色体畸变（间隙、染色单体断裂、染色单体交换和染色体断裂）的频率显著增加，但暴露于五价砷后未出现这种情况。当未受刺激的（G0期）淋巴细胞暴露于三价砷然后进行培养时，未发现这种效应。暴露于三价砷后，姐妹染色单体交换率也被发现有所增加。因此，结果表明三价砷比五价砷具有更强的基因毒性，并且砷主要在细胞分裂期间发挥其作用。
