Schardt C W, McLachlan S M, Matheson J, Smith B R
J Immunol Methods. 1982 Dec 17;55(2):155-68. doi: 10.1016/0022-1759(82)90028-x.
An enzyme-linked immunoassay (ELISA) for microsomal antibody is described. The method was found to be rapid, sensitive and precise and analysis of 115 serum samples showed good correlation between the ELISA and the conventional tanned red cell haemagglutination test. The presence of thyroglobulin antibody, rheumatoid factor, antinuclear factor or gastric parietal cell antibodies did not interfere in the microsomal antibody ELISA but some sera with mitochondrial antibody activity appeared to cause a non-specific effect. The ELISA was particularly useful for analysing microsomal antibody production by Hashimoto lymphocyte cultures and in some cases antibody synthesis could be studied in the absence of mitogen. The high capacity of the ELISA combined with its sensitivity suggest that it will be a valuable technique for studying microsomal autoantibody activity both in serum and in lymphocyte cultures.
本文描述了一种用于检测微粒体抗体的酶联免疫吸附测定（ELISA）方法。该方法快速、灵敏且精确，对115份血清样本的分析表明，ELISA与传统的鞣酸红细胞血凝试验之间具有良好的相关性。甲状腺球蛋白抗体、类风湿因子、抗核因子或胃壁细胞抗体的存在不会干扰微粒体抗体ELISA，但一些具有线粒体抗体活性的血清似乎会产生非特异性影响。ELISA对于分析桥本淋巴细胞培养物中微粒体抗体的产生特别有用，在某些情况下，无需有丝分裂原即可研究抗体合成。ELISA的高检测能力及其灵敏度表明，它将是研究血清和淋巴细胞培养物中微粒体自身抗体活性的一种有价值的技术。
