Broman K, Lauwers N, Stalon V, Wiame J M
J Bacteriol. 1978 Sep;135(3):920-7. doi: 10.1128/jb.135.3.920-927.1978.
Bacillus licheniformis has two pathways of arginine catabolism. In well-aerated cultures, the arginase route is present, and levels of catabolic ornithine carbamoyltransferase were low. An arginase pathway-deficient mutant, BL196, failed to grow on arginine as a nitrogen source under these conditions. In anaerobiosis, the wild type contained very low levels of arginase and ornithine transaminase. BL196 grew normally on glucose plus arginine in anaerobiosis and, like the wild type, had appreciable levels of catabolic transferase. Nitrate, like oxygen, repressed ornithine carbamoyltransferase and stimulated arginase synthesis. In aerobic cultures, arginase was repressed by glutamine in the presence of glucose, but not when the carbon-energy source was poor. In anaerobic cultures, ammonia repressed catabolic ornithine carbamoyltransferase, but glutamate and glutamine stimulated its synthesis. A second mutant, derived from BL196, retained the low arginase and ornithine transaminase levels of BL196 but produced high levels of deiminase pathway enzymes in the presence of oxygen.
地衣芽孢杆菌有两条精氨酸分解代谢途径。在通气良好的培养物中，存在精氨酸酶途径，分解代谢的鸟氨酸氨甲酰基转移酶水平较低。一种精氨酸酶途径缺陷型突变体BL196，在这些条件下不能以精氨酸作为氮源生长。在厌氧条件下，野生型含有极低水平的精氨酸酶和鸟氨酸转氨酶。BL196在厌氧条件下能在葡萄糖加精氨酸的培养基上正常生长，并且与野生型一样，具有相当水平的分解代谢转移酶。硝酸盐与氧气一样，会抑制鸟氨酸氨甲酰基转移酶并刺激精氨酸酶的合成。在需氧培养物中，在有葡萄糖存在的情况下，谷氨酰胺会抑制精氨酸酶，但当碳能源不足时则不会。在厌氧培养物中，氨会抑制分解代谢的鸟氨酸氨甲酰基转移酶，但谷氨酸和谷氨酰胺会刺激其合成。从BL196衍生出的第二个突变体，保留了BL196的低精氨酸酶和鸟氨酸转氨酶水平，但在有氧条件下会产生高水平的脱亚氨酶途径酶。
