Simon J P, Wargnies B, Stalon V
J Bacteriol. 1982 Jun;150(3):1085-90. doi: 10.1128/jb.150.3.1085-1090.1982.
The formation of the arginine deiminase pathway enzymes in Streptococcus faecalis ATCC 11700 was investigated. The addition of arginine to growing cells resulted in the coinduction of arginine diminase (EC 3.5.3.6), ornithine carbamoyltransferase (EC 2.1.3.3), and carbamate kinase (EC 2.7.2.3). Growth on glucose-arginine or on glucose-fumarate-arginine produced a decrease in the specific activity of the arginine fermentation system. Aeration had a weak repressing effect on the arginine deiminase pathway enzymes in cells growing on arginine as the only added substrate. By contrast, depending on the growth phase, a marked repression of the pathway by oxygen was observed in cells growing on glucose-arginine. We hypothesize that, in S. faecalis, the ATP pool is an important signal in the regulation of the arginine deiminase pathway. Mutants unable to utilize arginine as an energy source, isolated from the wild type, exhibited four distinct phenotypes. In group I the three enzymes of the arginine deiminase pathway were present and probably affected in the arginine uptake system. Group II mutants had no detectable arginine deiminase, whereas group III mutants had low levels of ornithine carbamoyltransferase. Group IV mutants were defective for all three enzymes of the pathway.
对粪肠球菌ATCC 11700中精氨酸脱亚胺酶途径酶的形成进行了研究。向生长中的细胞添加精氨酸会导致精氨酸脱亚胺酶（EC 3.5.3.6）、鸟氨酸氨甲酰基转移酶（EC 2.1.3.3）和氨基甲酸激酶（EC 2.7.2.3）的共诱导。在葡萄糖 - 精氨酸或葡萄糖 - 富马酸 - 精氨酸上生长会导致精氨酸发酵系统的比活性降低。通气对以精氨酸作为唯一添加底物生长的细胞中的精氨酸脱亚胺酶途径酶具有较弱的抑制作用。相比之下，根据生长阶段，在以葡萄糖 - 精氨酸生长的细胞中观察到氧气对该途径有明显的抑制作用。我们推测，在粪肠球菌中，ATP库是精氨酸脱亚胺酶途径调节中的一个重要信号。从野生型中分离出的无法利用精氨酸作为能量来源的突变体表现出四种不同的表型。在第一组中，精氨酸脱亚胺酶途径的三种酶都存在，并且精氨酸摄取系统可能受到影响。第二组突变体没有可检测到的精氨酸脱亚胺酶，而第三组突变体鸟氨酸氨甲酰基转移酶水平较低。第四组突变体该途径的所有三种酶都有缺陷。
